Staff are reapplying for 100 'new' roles, believed to be at lower rates of pay.
B&Q refused to comment on what gardening roles are going as part of a net loss of staff across the whole business, saying: "We can't give details which staff are affected."
B&Q parent company Kingfisher saw pre-tax profit plummet 15.5 per cent to £371 million in its first half of 2012. Sales of seasonal products fell seven per cent for the 26 weeks ending July 28 2012 - around £30m.
CEO Ian Cheshire said then (HW 28 September): "It leads us to think about the weather sensitivity of the business -should we shift the model away from weather-driven categories?
"B&Q starts from a position of being the number one garden centre by volume. It's a footfall driver. Our challenge is to adjust the mix so it's less susceptible."
The chain wants to move to be an "omnichannel" retailer rather than opening new stores.