An additional 1,500 new plants have been added to the latest edition. Now more than 12,000 plants - in more than 650 genera - are described in detail.
The collaboration ensures all plant names correspond directly with those in RHS Plant Finder. Readers of the Hillier Manual will now be able to cross refer to entries in RHS Plant Finder.
RHS principal botanist James Armitage said: "The RHS has drawn on its unique resources to harmonise the 10,500 entries in the previous Hillier Manual with those used in RHS Plant Finder.
We've written over 1,500 new entries and updated information on tree heights, recent introductions of plants from the wild into gardens and plants holding the RHS Award of Garden Merit (AGM)."
Hillier managing director Andrew McIndoe said: "I am delighted to see a new edition of The Hillier Manual of Trees and Shrubs published in the 150th year of Hillier Nurseries. The Manual is such an important part of the heritage of Hillier that it is wonderful to see it progress to the next stage in its history in partnership with the Royal Horticultural Society.
"This new edition is a true collaboration between John Hillier, Roy Lancaster, and RHS botanists. We are certain that this will ensure that it remains the definitive reference on trees and shrubs for horticulturists, both amateur and professional."
The Hillier Manual of Trees and Shrubs is available from 28 March 2014 at £19.99.