The £10 per day charge will be brought in from mid to late April but Friends of Kew, Wakehurst Place season ticket holders and those who purchase a day ticket to enter Wakehurst will continue to park for free.
The Royal Botanic Gardens, Kew (RBGK) leases the gardens, woodlands and mansion at Wakehurst Place from the National Trust but does not see entry revenue from 80 per cent of visitors.
So despite Wakehurst Place being one of the trust’s "most popular properties", the two organisations say the new fee is the only way they can afford to maintain standards.
In a joint statement released by chairman of the National Trust Sir Simon Jenkins and Kew chairman Marcus Agius, the partners said they had been working together to find a sustainable future for Kew at Wakehurst Place but revealed they needed to raise more money.
The Royal Botanic Gardens, Kew currently maintains the gardens, woodlands and the mansion at Wakehurst Place.
"Despite being run efficiently and effectively, the net cost to Kew of running Wakehurst Place is such that we have reached a point where greater income is needed if we are to continue our outstanding horticultural standards and the wonderful visitor experience," the statement read.
"We have agreed that the best way to raise this much needed income is through the introduction of car parking charges in April 2014.
Car drivers will pay £2 for the first hour, £5 for two hours, and £10 per day but coaches and minibuses will park for free.
Wakehurst holds the world’s largest seed conservation project and is a centre for research as well as offering woodland, lakes, formal gardens and an Elizabeth property to visitors.
The NT and RBGK are holding a series of meetings for people who want to discuss the charges.
These will take place between 10.30am and 11.30am and 1pm and 2pm on 17 and 24 March in the mansion dining room. Email email@example.com or telephone 01444 894067 if you wish to attend.