The ad agency for Japanese Knotweed Solutions fell foul of the publication by using a photo of the middle-finger salute and a "Bring it on" strapline.
This was too much for the environment magazine, which refused to run the ad from Blueleaf, based near the Manchester head office of its knotweed client.
Studio manager Duncan Watts said his agency had been briefed by Japanese Knotweed Solutions to write a "hard-hitting" advert for the "most invasive plant in Britain".
"The advert needed to be impactful and reflect the client's no-nonsense approach to dealing with the problem," he said. "It is brash and stands out but is intelligent."
The raised finger, which can be seen at www.jksl.com/ad, emphasised the fact that it can take just a piece of weed rhizome the size of a fingernail to cause devastation.