The verbal battle of the sexes will form an opening salvo to mark an exhibition called Celebrating 20th Century Women Gardeners.
The debate, at Montacute House in Somerset, will be between veteran BBC horticulturists Stefan Buczacki and Anne Swithinbank.
Both will try to identify male and female gardening styles in a head-to-head clash chaired by gardening journalist Tim Richardson.
The exhibition will be held at three National Trust gardens from June to October - Barrington, Tintinhull and Montacute. It will highlight the profound influence Gertrude Jekyll, Phyllis Reiss and Vita Sackville-West had on the three gardens.
"During the early 20th century, the contribution made by these great women to gardening began to gain greater acknowledgement," said the National Trust.
The exhibition will include installations and sculpture to capture the sensory impact each woman brought and to illustrate their individual personalities, styles and designs.
Tickets to the Battle of the Secateurs debate on 8 June cost £20 (including lunch). Call 01935 823289 to book.