Ben Gregory will cycle more than 385 kilometres from April 30 until May 3 in aid of Cyclists Fighting Cancer, The Cystic Fibrosis Trust and The Yeovil Community Trust.
Gregory, who is 23-years-old and originally from Yeovil in Somerset, said: "This is going to be a massive challenge for me as I am still relatively new to cycling and I will have to cycle up to 80 miles a day.
"My brother Sam and I decided to do the challenge because the charities are particularly pertinent to us as like so many families, my family has been heavily affected by cancer.
"I was also keen to support the Cystic Fibrosis Trust as, like many another diseases, there is currently no cure and only continued support to the charity will help them to research advanced treatments to improve the lives of those who unfortunately have this disease. The final charity is The Yeovil Community Trust, which supports my home town, local community and I feel it is good to give something back to the community I grew up in.
"Any donations will be greatly appreciated and will go a long way to help these fantastic organisations. My brother and I hope to raise at least £1,000."
The event is organised by supporters of Yeovil Town Football Club and this is the third successive year they have hosted a sports charity event. The challenge will also be completed by 108 men and women from Yeovil and the surrounding area and all the money donated will be directly split between the three chosen charities.
The challenge will start in Paris on April 30 and the day will finish in Evreux via Merey and Ivry-la Bataille. On the second day the team will finish in Ouistreham near Caen ready for the participants to catch the ferry. They will then have an overnight crossing to Portsmouth.
Day three will start in Portsmouth and end in Bournemouth via Southampton and the New Forest and on the final day the group will ride from Bournemouth to finish in Yeovil.
Adam Dunnett, sales and marketing director at Wyevale Nurseries, said: "We would like to wish Ben and Sam the best of luck for the ride. We have also sponsored Ben £100 as they are raising money for some great causes. We hope the horticultural industry will get behind them too and show its support."
The Cystic Fibrosis Trust started in 1964 and is dedicated to promoting excellence in research and clinical care, as well as providing practical support and advice to people with cystic fibrosis and their families.