The plan states: "A site on Graveley Road, currently already in use as a garden centre, is identified to accommodate such a large store.
"Although on the northern edge of the borough, this store will be well located in respect of the new urban extension North of Stevenage."
Wyevale Garden Centres did not comment.
Stevenage council says new food stores will be needed by 2031.
Meanwhile, Brentwood Borough Council has identified Ingatestone Garden Centre in Essex, which is currently closed, as a potential development sites.
Ingatestone Garden Centre, in Chelmsford, Essex, had the gates closed to staff last Monday and there is now to be an auction off of stock. Owner James Harding said the centre had been losing money.
The plan shows in addition to the 2,500 homes developers have proposed at Dunton Garden Suburb, 500 are set for industrial estates in West Horndon and between 500 and 600 at Officers' Meadow off Alexander Lane in Shenfield.
There are also 216 proposed on land off Doddinghurst Road, 130 on land east of Nags Head Lane, between 150 and 250 on land at Honeypot Lane, 68 for the council depot in Warley's The Drive, 60 at Ingatestone Garden Centre and 50 at Warley Training Centre.
A similar situation arose at Coblands last year, where the council designated the site as potential housing.
Meanwhile, a garden centre and antiques business is to close at the end of February.
Rockwood Garden Centre in Grange Moor, West Yorkshire, has seen a drop in trade and will shut at the end of the month.
The garden centre and cafe, which previously had an antiques centre, art gallery and studio space upstairs, opened in April, 2012.