Garden Organic’s Ryton Gardens is closing its garden centre and café to make way for an interactive education centre.
The move was prompted by leaseholder and operator Webbs Garden Centres ending the lease early using the release clause.
Ryton was an organic-only garden centre which Webbs took over in 2010 and was the only UK centre where organic produce was "first choice".
The charity’s chairman of trustees Roger Key said trustees now want to "concentrate on the core objectives of the charity" at the 4ha gardens in Ryton, Coventry.
A library, displays about Garden Organic’s work, organic gardening demonstrations and experiments in gardens are all planned. The six-strong professional gardening team will also redesign and rework the demonstration gardens.
Webbs chairman Ed Webb said: "Webbs gave notice to Garden Organic on ending its lease of the shop and cafe at Ryton Gardens in January this year in order to focus more on its successful stores at Wychbold and West Hagley.
"Garden Organic has announced exciting plans to re-launch a new education and resource centre at Ryton Gardens and we wish them every success for the future."
"We would like to thank the dedicated and hardworking Webbs Ryton Gardens team and had been striving for the operations to transfer to Garden Organic. Unfortunately Garden Organic has made the decision not to operate the shop and cafe in the future. We are in active discussions with staff looking at alternative employment within Webbs and will provide every support possible including, where appropriate, practical help with finding alternative employment."