Lamberhurst-based Twig Trading scooped the prize in the Kent Invicta Chamber of Commerce Business Awards 2010. Managing director David Eyre was also a runner-up in the awards' entrepreneur of the year category.
Following growth in the business since 2002, Eyre now plans to take on four extra staff in the coming months. The firm added four staff to its team of six last year to carry out the work at Thanet Earth.
Eyre said: "It is an amazing feeling to have won the business of the year accolade. I am very proud of the tireless hard work the team put in. These awards recognise our values and the importance we place on conservation when carrying out landscaping and countryside management projects."
Eyre founded the company in 2002. Over the past year, the team planted 19,100 trees.
Twig has almost doubled in size year-on-year for the past three years. Eyre employs 10 people and expects to take on four more staff in the coming months to carry out work on education projects.
The firm has been designing new grounds for Sissinghurst Primary School, including performance areas, outdoor classrooms, wildlife walks and vegetable plots.
Twig has also created a sensory garden at a school for children with additional needs in Gravesend, while in Berkshire it has been building a beach-themed play area. In Essex, the team is developing a wildlife and vegetable and flower growing area for a primary school.