The annual festival, now in its third year, will run from 25 November to 2 January. The mile-long Christmas trail will this year celebrate Kew's part in the history of the modern Christmas tree.
Queen Charlotte, a founder of Kew Gardens, first dressed branches of a Christmas tree in one of the largest rooms at Kew Palace for all to enjoy in the 1790s.
Tree canopies will be drenched in Christmas lights while the fountains in front of the Palm House will light up and dance. There will also be a "fairytale avenue" of winter flora including snowdrops and Christmas roses, and a scented "Fire Garden".
The trail opens at 5pm and closes at 10pm, with visits timed to 30 minutes to walk the trail.
A Kew spokesperson said the Christmas festival was producing "extremely healthy ticket sales" and had contributed to increasing visitor numbers over the past couple of years.