More than £500,000 of container-grown trees, shrubs and a range of other plants are being offered for sale by online auction.
On 23 November, local garden centres, landscapers and an online garden centre viewed the nursery stock with the view to buy today.
John Woods, a £6m turnover business, formed in 2007 as part of a management buyout from Notcutts Nursery.
The business had run into severe financial problems after several years of difficult trading conditions, and announced a restructuring and creditors voluntary arrangement (CVA) in November 2014.
The nurseries produced more than 1.3 million hardy plants a year on its 65-acre site in Woodbridge, Suffolk. Its 1,500-line product range includes shrubs, perennials, roses, ericaceous plants, climbers, trees and fruit.
The online auction is being organised by Eddisons. See www.eddisons.com/online
Landlord William Notcutt said: "I’m very sad that it has come to an end. It brought back memories of the management buy out in 2007 and the end of Notcutts Nurseries, and I feel very sad for the staff."
Notcutts company chairman Nicky Dulieu said: "Notcutts have been a supporter of John Woods Nurseries for many years and we are very sorry to hear the news."