Several UK offices including Milton Keynes had been earmarked for 149 redundancies in the support function sector. A consultation process of 45 days will begin on April 18.
The redundancies are all part of the restructuring of Homebase, which has included several directors going after Australia-based Bunnings owner Wesfarmers bought the company.
There are around 800 staff in head offices, including buyers, marketing and other support staff.
Homebase said: "Earlier this month we updated our team about a proposed restructure of all Store Support Functions. Obviously we will need to speak to the people whose jobs will be affected first and it would not be appropriate to give details while we are involved in a consultation process.
"What we can say is that we are not simply making across the board changes. Rather, we are reshaping the support resource to allow us to help the stores in the best manner possible.
"We have committed to our team that we will move forward as quickly as we can and that we will treat every team member affected generously and with respect."
Homebase said there are no current plans to end the Garden Academy, where 30 students joined this month and 40 in November 2015.
The 265 Homebase stores are to take on the Bunnings name.
Wesfarmers will announce sales figures from its retailers this week, with home stores doing best and an expected overall sales rise of 5.3 per cent.
Wesfarmers chief executive Richard Goyder has backed plans to pay Homebase staff aged under 25 the National Minimum Wage, as proposed by new Bunnings UK chief Peter Davis.