Jon Kemp was Homebase trading director DIY and garden and had been at Home Retail Group for 20 years.
Andy Goddard was horticulture category manager at Homebase.
Jane Templar, who was Homebase plant buying manager has also gone. Owners Wesfarmers confirmed their departures this week.
Paul Emslie moved to Wyevale Garden Centres as trading and marketing director this month having been commercial director at Homebase.
Their replacement have yet to be announced.
Wesfarmers bought Homebase in January for £340m. Many directors of Homebase, including managing director Echo Lu, have since left.
Wesfarmers said it will announce further plans for Homebase this month. It did not say who were replacing the staff who have left.