The petition, launched in March by Sarah Bentley of community food kitchen Made in Hackney, has garnered more than 1,000 signatures. It calls for the London borough's council to "stop spraying all toxic chemicals in Hackney streets, parks, schools and public space".
It says children should be able to play safely in the borough's parks - including burying their faces in the dirt - without fear they are being exposed to "glyphosate and other toxic nasties".
Bentley warned the public were also at risk of being sprayed by weed control trucks while walking in the streets, claiming it has happened to her twice including once while heavily pregnant.
The campaigners also want signs erected in London Fields' wildflower meadow warning that glyphosate has been used there. But the council has decided not to add the signage, according to the Hackney Citizen.
Fashion designer Katharine Hamnett first began campaigning in 2014 to stop glyphosate being sprayed in Hackney's parks, with her petition raising more than 3,000 signatures. Hackney Council chose at that time to continue using glyphosate, citing Defra, the HSE and the EU's stance that the chemical was not harmful to the public.
Roundup was also pulled from the shelves of some local garden centres last year after pressure from Hackney residents.