The garden centres involved will be officially opening their new pet shops during April as part of National Pet Month.
Melanie Malin, Category Manager for Animal Care at The Garden Centre Group, said: "Staff will be on hand to give customers free advice on pet care throughout April and beyond.
"They have been fully trained in product knowledge and will be able to assist people with their pet-related questions.
"The new areas will include leading cat and dog food brands such as James Wellbeloved, Hills, Royal Canin and Pro Plan and will offer a wide range of accessories comprising of pet beds, collars, scratching posts, carriers, kennels and health and well-being products.
"Small animals such as rabbits and guinea pigs will be catered for with brands including Burgess Excel and Supreme animal feeds, a range of bedding, accessories and British-made hutches. In addition, there is a brand new selection of chicken products including the Countryside range of chicken feeds, products for health and well-being, a range of housing and even some treats for your garden hens.
"The garden centres will also have a range of on-going promotional deals on various products, while stocks last."
The 31 garden centres that have launched new pet shops are:
1. Albrighton Garden Centre
2. Blooms Solihull Garden Centre
3. Bold Heath Garden Centre
4. Bridgemere Nursery & Garden World
5. Bury St Edmunds Garden Centre
6. Canterbury Chartham Garden Centre
7. Crowland Outlet Garden Centre
8. Fair Oak Garden Centre
9. Findon Garden Centre
10. Hamstreet Garden Centre
11. Heathlands Garden Centre
12. Heighley Gate Nursery & Garden Centre
13. Hereford Garden Centre
14. Keynsham Garden Centre
15. Louth Garden Centre
16. Marple Garden Centre
17. Osterley Garden Centre
18. Par Garden Centre
19. Poppleton Garden Centre
20. Pulborough Garden Centre
21. Ramsgate Garden Centre
22. Shirley Garden Centre
23. Stanway Garden Centre
24. Stockton Garden Centre
25. Wimborne Garden Centre
26. Woodlands Nursery & Garden Centre
27. Worthing Ferring Garden Centre
28. Wyevale Lelant Garden Centre
29. Wyevale Telford Garden Centre
30. Wyevale Woburn Sands Garden Centre.