Managing director Andy King said: "As we celebrate 150 years of gardening, it makes sense to partner with a charity that shares our passion and recognises the power of getting things growing.
"In June, we’ll be holding a special event across our 35 stores to mark the occasion with the proceeds going to the appointed charity partner."
Dobbies has raised £350,000 for local and national charities in the last financial year. Through various fundraising initiatives and anniversary events, Dobbies aims to raise £50,000 for the chosen charity anniversary partner.
The deadline for applications is 20 February 2015. Three finalist charities will be selected and public voting via social media and in-store will launch on 9 March 2015. The anniversary charity partner will be announced after voting closes on 23 March 2015.
Dobbies will be at the Garden Press Event on 12 February. It is opening a stand-alone cafe at its Dundee centre this spring. Plans for an East Kilbride centre were shelved last month.
Retail analysts have suggested Tesco-owned Dobbies might be bought by Wyevale Garden Centres, but neither party has commented on any moves.