The 2.4ha Cupar garden centre was sold by John and Carol Hodson, who are retiring. Colin and Mandy Barrie own Caulders Garden Centres.
It has a turnover of more than £1m plus a coffee shop, which was operated as a concession but will now be operated by the garden centre.
The family-owned independent business now employs around 140 staff.
Kirkintilloch was opened in 2002, followed by the purpose-built Braidbar centre in 2009 and Erskine in 2014.
Cupar Garden Centre is situated near the Eden Valley Business Park on the A91.
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