Designed by award-winning firms Gustafson Porter and Gustafson Guthrie Nichol, the installation - Towards Paradise - has been created in the overgrown grounds of the former Church of the Virgins.
The garden is composed of three main spaces linked by pathways, which tell a story of past, present and future.
Gustafson Porter director Neil Porter said: "We believe landscape architecture is a greatly neglected subject, so we are hugely excited by the opportunity of engaging with visitors at the Biennale."
The Venice Biennale is a major art and cultural exhibition which dates from 1895. As part of the Biennale, an international architecture exhibition - Out There: Architecture Beyond Building, is taking place from 14 September to 23 November.